Job ID: 122764

POSTDOCTORAL POSITION ON LOCAL TRANSLATION IN ASTROCYTES

Position: Post-doctoral Position

Deadline: 1 August 2025

Employment Start Date: 1 September 2025

Contract Length: 1 year to start

City: Paris

Country: France

Institution: CIRB Collège de France

Department: Neurosciences

Description:

POSTDOCTORAL POSITION ON LOCAL TRANSLATION IN ASTROCYTES

Center for interdisciplinary research in Biology, Collège de France, Paris.

Team Physiology and physiopathology of the gliovascular unit.

https://www.college-de-france.fr/site/en-cirb/cohen-salmon.htm

Applications are invited for a postdoctoral position to study local translation in astrocytes. The focus of the project will be to characterize local translation in astrocytes to understand its contribution to cell compartmentalization in physiology and physiopathology. Our research combines molecular approaches to characterize mRNAs and the translation machinery in astrocyte interfaces during brain development and in the adult in mouse models by:

1) Molecular approaches: Translating affinity ribosome purification, Ribosome profiling, RNA and protein immunoprecipitation, RNAseq, Mass spectrometry, qPCR.

2) Imaging approaches: Fluorescent in situ hybridization, FUNCAT, FUNCAT-PLA, immunofluorescence, 3D reconstruction of astrocytes and their interfaces and image quantification.

Candidates must have a PhD degree in neuroscience and a solid expertise in translation mechanisms. The ability to work both independently and cooperatively within a team is essential.

The appointment is currently for 1 year and candidates will have to apply to additional funding opportunities. The position is available starting from now.

Candidates should send their CV, motivation letter, list of publications, and letters of reference to

martine.cohen-salmon@college-de-france.fr.

Avila-Gutierrez, K., Slaoui, L., Alvear-Perez, R., Kozlowski, E., Oudart, M., Augustin, E., Claveau, C., Mailly, P., Monnet, H., Mignon, V., et al. (2024). Dynamic local mRNA localization and translation occurs during the postnatal molecular maturation of perivascular astrocytic processes. Glia 72, 777-793.

Boulay, A.C., Mazare, N., Saubamea, B., and Cohen-Salmon, M. (2019). Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain-Vascular Interface. Methods Mol Biol 1938, 105-116.

Boulay, A.C., Saubaméa, B., Adam, N., Chasseigneaux, S., Mazaré, N., Gilbert, A., Bahin, M., Bastianelli, L., Blugeon, C., Perrin, S., et al. (2017). Translation in astrocyte distal processes sets molecular heterogeneity at the gliovascular interface. Cell Discovery 3, 17005.

Mazare, N., Gilbert, A., Boulay, A.C., Rouach, N., and Cohen-Salmon, M. (2018). Connexin 30 is expressed in a subtype of mouse brain pericytes. Brain Struct Funct 223, 1017-1024.

Mazare, N., Oudart, M., Cheung, G., Boulay, A.C., and Cohen-Salmon, M. (2020).Immunoprecipitation of Ribosome-Bound mRNAs from Astrocytic Perisynaptic Processes of the Mouse Hippocampus. STAR Protoc 1, 100198.

Mazare, N., Oudart, M., and Cohen-Salmon, M. (2021). Local translation in perisynaptic and perivascular astrocytic processes – a means to ensure astrocyte molecular and functional polarity? J Cell Sci 134.

Mazare, N., Oudart, M., Moulard, J., Cheung, G., Tortuyaux, R., Mailly, P., Mazaud, D., Bemelmans, A.P., Boulay, A.C., Blugeon, C., et al. (2020). Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning. Cell Rep 32, 108076.

Oudart, M., Avila-Gutierrez, K., Moch, C., Dossi, E., Milior, G., Boulay, A.C., Gaudey, M., Moulard, J., Lombard, B., Loew, D., et al. (2023). The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activity. Cell Rep 42, 112456.

Oudart, M., Tortuyaux, R., Mailly, P., Mazare, N., Boulay, A.C., and Cohen-Salmon, M. (2020). AstroDot – a new method for studying the spatial distribution of mRNA in astrocytes. J Cell Sci 133.

Tortuyaux, R., Avila-Gutierrez, K., Oudart, M., Mazare, N., Mailly, P., Deschemin, J.C., Vaulont, S., Escartin, C., and Cohen-Salmon, M. (2023). Physiopathological changes of ferritin mRNA density and distribution in hippocampal astrocytes in the mouse brain. J Neurochem 164, 847-857.