Job ID: 118093

PhD Student Position at the Ambrozkiewicz Lab, Charité-Universitätsmedizin Berlin, Germany

Position: Ph.D. Student

Deadline: 5 April 2024

Employment Start Date: 6 May 2024

Contract Length: 3 years

City: Berlin

Country: Germany

Institution: Charité-Universitätsmedizin Berlin

Department: Institute of Cell Biology and Neurobiology

Description:

We are looking for a motivated PhD candidate, with a strong background in biochemistry, cell biology or neuroscience, to join our research group at the Charité. This position is initially funded for 3 years and funded according to German compensation schemes, TVöD.

As a PhD student in the team, you will join the vibrant Berlin Neuroscience Campus and have the access to the state-of-the-art imaging and other core facilities, mouse house and well-equipped wet lab. You will have an excellent opportunity to work within our interdisciplinary team engaged in many collaborative projects that employ transcriptomics, proteomics, in vivo genetic manipulations, organoids, among others.

The project addresses the fundamental questions in the are of developmental neurosciences. We are aiming to understand the role of ubiquitin signaling in the developing brain, particularly in the context of human neurogenetic diseases. We will investigate the molecular landscapes in the neuronal stem cells and establish the function of E3 ubiquitin ligases in maintaining the proteomes across neuronal lineages. Using mouse as a model organism, we will translate the biochemical findings into the assembly of the cerebral cortex during embryogenesis.

Key tasks and responsibilities:

  • Develop cell-based biochemical assays for studying of the enzymatic activities of E3 ubiquitin ligases,
  • Basic expression pattern characterization using RNA hybridization and protein-detecting methods in the brain for E3s,
  • Developmental phenotyping using in utero electroporation (conditional knock-out and knock-in lines, CRISPR-Cas9, sh-RNA),
  • Establishment of proteomic pipelines (substrate identification, proteome quantification etc.),
  • Advanced imaging protocols using mouse brain samples,
  • Integration into the internal and external collaboration of our lab,
  • Contribution to the inclusive and diverse work culture within our group.

Preferred qualifications:

  • Commitment and motivation to pursue a project in basic research,
  • Master’s (or equivalent) in biochemistry, biology, bioengineering, or neuroscience,
  • Experience in statistical analysis and data science,
  • Proficiency in English,
  • Quick learning and ability to work independently.

To apply, please submit the CV and a motivation letter, as well as the names of two-three referees to mateusz-cyryl.ambrozkiewicz@charite.de. Application deadline April 5th, 2024.